PRF Protocols: The Art and Science of Regenerative Dentistry
- Carlie Amore
- Dec 30, 2025
- 4 min read
Nature’s Blueprint for Healing
Every time we’re injured, our body knows exactly what to do.Platelets rush in, fibrin forms a scaffold, and growth factors signal new tissue to grow.
Platelet-Rich Fibrin (PRF) captures and amplifies that process — naturally, safely, and elegantly.
At Amore Dentistry, we use PRF as the foundation for every biologic surgery: extractions, implants, bone grafts, sinus lifts, and even aesthetic healing.
“When we follow biology, healing stops being complicated — it becomes inevitable.”
The Science Behind PRF
PRF is derived from your own blood — no additives, no anticoagulants.We draw a small amount (usually 10–60 mL), then immediately centrifuge it to separate the components by density.
Red Blood Cells (RBCs): Settle at the bottom
Platelet-Rich Fibrin (PRF) Layer: Middle, gel-like golden zone
Plasma (PPP): Top clear layer
That golden layer — rich in platelets, leukocytes, and fibrin — becomes the matrix for natural tissue regeneration.
Unlike PRP (platelet-rich plasma), which uses anticoagulants and creates a liquid fraction, PRF forms a solid fibrin clot that slowly releases growth factors for up to 10–14 days.
This creates a time-released healing environment — a living biomaterial that acts as both scaffold and signal.
Fibrin Architecture: The Healing Scaffold
What makes PRF remarkable isn’t just what’s in it — it’s how it’s structured.
During centrifugation, the fibrin fibers polymerize naturally, creating a dense, elastic mesh that traps:
Platelets (PDGF, TGF-β, VEGF, IGF)
White blood cells (immune regulation)
Circulating stem cells
This three-dimensional structure guides cell migration, angiogenesis, and collagen deposition.
Think of it like the body’s original 3D printer — laying down the blueprint for tissue to rebuild itself.
Centrifugation: Speed, Force, and Time
The magic of PRF lies in respecting biology’s rhythm.Too much force, and the fragile platelets break.Too little, and the separation fails.
Following Dr. Ghanaati’s low-speed centrifugation concept (LSCC) ensures optimal cell yield and fibrin density.
Here’s a simplified version of the protocols we use at Amore Dentistry:
PRF Type | Tube | Speed | Time | Use |
A-PRF+ | Red or glass | 1,300 rpm (~200g) | 8 min | Membranes, sockets |
i-PRF | Plastic | 700 rpm (~60g) | 3 min | Injectable, graft mixing |
T-PRF | Titanium | 2,700 rpm (~400g) | 12 min | Extended fibrin stability |
These gentle settings preserve platelets, growth factors, and fibrin elasticity.
Once processed, the PRF is compressed into membranes or injected into graft sites — depending on the clinical need.
Biologic Mechanisms: Growth Factors in Action
PRF supports wound healing through a synergistic cascade of growth factors:
Growth Factor | Function |
PDGF (Platelet-Derived Growth Factor) | Stimulates fibroblast and collagen production |
TGF-β (Transforming Growth Factor) | Regulates matrix formation and immune balance |
VEGF (Vascular Endothelial Growth Factor) | Promotes new blood vessel growth |
IGF (Insulin-like Growth Factor) | Encourages bone and soft-tissue regeneration |
EGF (Epidermal Growth Factor) | Accelerates epithelial closure |
This cocktail of signals works in perfect biological harmony — no drugs, no chemicals, just your body’s own intelligence amplified.
The Guided Open Wound Healing Concept
As taught by Dr. Shahram Ghanaati and the Frankfurt research group, PRF supports guided open wound healing — a biologic alternative to primary closure.
Instead of pulling tissue tightly with sutures, we leave the site open under a stable PRF cover.This encourages secondary intention healing through oxygenation, drainage, and natural granulation.
The benefits:
Reduced post-op pain
Less swelling
Increased vascularization
Faster epithelialization
Cleaner bone fill
This approach honors the body’s timeline — not the industry’s.
Synergy with Ozone and Photobiomodulation
PRF doesn’t work alone — it thrives in a biologic ecosystem.
Ozone Therapy: Ozone disinfects, oxygenates, and stimulates immune modulation before PRF placement — ensuring the wound bed is clean and primed.
Photobiomodulation (PBM): Red and near-infrared light increase mitochondrial ATP production and enhance fibroblast proliferation — complementing PRF’s growth factor release.
Together, these therapies transform surgical recovery into regeneration.
Clinical Applications at Amore Dentistry
PRF is versatile — we use it across nearly every surgical and restorative procedure:
Extractions (Cavitation and Surgical): Fibrin plugs stabilize clot and prevent dry socket.
Zirconia Implants: Membranes line osteotomies for immediate angiogenesis and improved osseointegration.
Bone Grafts + Sinus Lifts: i-PRF blended with graft particles (sticky bone) ensures stable volume and cellular vitality.
Gingival and Soft-Tissue Grafting: Improves tissue tone, color, and healing speed.
Facial Aesthetics / PRF Facials: Stimulates dermal rejuvenation through collagen remodeling — healing from the inside out.
A Patient Story: The Natural Way to Heal
One of my patients had two upper molars removed after chronic infection.We used ozone lavage, placed A-PRF+ membranes, and performed low-level light therapy post-op.
Within 48 hours, there was zero swelling and minimal tenderness.At 6 weeks, the socket was 95% filled with new tissue — no grafts, no complications.
She said, “It felt like my body did all the work — you just guided it.”
Exactly. That’s the art and science of biologic dentistry.
Key Takeaways
PRF is 100% autologous — no additives, no chemicals. Low-speed centrifugation preserves cell vitality. Growth factors guide regeneration and immune modulation. Synergistic with ozone and PBM for optimal healing. PRF supports guided open wound healing with less trauma.
Conclusion: Partnering with Biology
In a world obsessed with faster, harder, and synthetic, PRF reminds us that the body already knows how to heal — we just need to support it.
It’s art because every fibrin clot is unique.It’s science because every molecule has purpose.
At Amore Dentistry, we don’t close wounds — we guide them.We don’t fight inflammation — we regulate it.And we don’t dominate biology — we dance with it.
PRF is proof that the best medicine has always been inside us.
References
Ghanaati S, et al. “Advanced platelet-rich fibrin: A new concept for cell-based tissue engineering.” Clin Oral Investig. 2014. PubMed
Miron RJ, Choukroun J. Platelet Rich Fibrin in Regenerative Dentistry: Biological Background and Clinical Indications. Wiley Blackwell, 2017.
Ghanaati S. “Low speed centrifugation concept for optimized PRF.” J Oral Implantol. 2018. PubMed
Dohan Ehrenfest DM, et al. “Classification of platelet concentrates: From pure PRP to leukocyte- and PRF.” Trends Biotechnol. 2009. PubMed
IAOMT Clinical Protocol Guide. “Autologous Fibrin in Guided Wound Healing.” 2023.
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